Trapped ion mobility spectrometry (TIMS) using parallel accumulation serial fragmentation (PASEF®) is an advanced analytical technique that offers several advantages in mass spectrometry (MS)-based lipidomics. TIMS provides an additional dimension of separation to mass spectrometry and accurate collision cross-section (CCS) measurements for ions, aiding in the structural characterization of molecules. This is especially valuable in lipidomics for identifying and distinguishing isomeric or structurally similar compounds. On the other hand, PASEF technology allows for fast and efficient data acquisition by accumulating ions in parallel and then serially fragmenting them. This accelerates the analysis process and improves throughput, making it suitable for high-throughput applications. Moreover, the combination of TIMS and PASEF reduces co-elution and ion coalescence issues, leading to cleaner and more interpretable mass spectra. This results in higher data quality and more confident iden...

Denti, V., Serrao, S., Bossi, E., Paglia, G. (2025). UHPLC-TIMS-PASEF®-MS for Lipidomics: From Theory to Practice. In O. Deda, H.G. Gika, I.D. Wilson (a cura di), Metabolic Profiling Methods and Protocols (pp. 221-237). Springer Nature [10.1007/978-1-0716-4334-1_12].

UHPLC-TIMS-PASEF®-MS for Lipidomics: From Theory to Practice

Denti V.;Serrao S.;Bossi E.;Paglia G.
2025

Abstract

Trapped ion mobility spectrometry (TIMS) using parallel accumulation serial fragmentation (PASEF®) is an advanced analytical technique that offers several advantages in mass spectrometry (MS)-based lipidomics. TIMS provides an additional dimension of separation to mass spectrometry and accurate collision cross-section (CCS) measurements for ions, aiding in the structural characterization of molecules. This is especially valuable in lipidomics for identifying and distinguishing isomeric or structurally similar compounds. On the other hand, PASEF technology allows for fast and efficient data acquisition by accumulating ions in parallel and then serially fragmenting them. This accelerates the analysis process and improves throughput, making it suitable for high-throughput applications. Moreover, the combination of TIMS and PASEF reduces co-elution and ion coalescence issues, leading to cleaner and more interpretable mass spectra. This results in higher data quality and more confident iden...
Capitolo o saggio
Ion mobility; Lipidomics; Mass spectrometry; PASEF; TIMS;
English
Metabolic Profiling Methods and Protocols
Deda, O; Gika, HG; Wilson, ID
2025
9781071643334
2891 MIMB
Springer Nature
221
237
Denti, V., Serrao, S., Bossi, E., Paglia, G. (2025). UHPLC-TIMS-PASEF®-MS for Lipidomics: From Theory to Practice. In O. Deda, H.G. Gika, I.D. Wilson (a cura di), Metabolic Profiling Methods and Protocols (pp. 221-237). Springer Nature [10.1007/978-1-0716-4334-1_12].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/541801
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