Mass cytometry is now a well-established method that enables the measurement of 40–50 markers (generally proteins but transcripts are also possible) in single cells. Analytes are detected via antibodies tagged with heavy metal and detected by using a time-of-flight mass spectrometer. Over the past decade, mass cytometry has proven to be a valuable method for immunophenotyping hematopoietic cells with remarkable precision in both healthy and malignant scenarios. This chapter explains in detail how to profile hematopoietic cells by using this high-dimensional multiplexed approach.
Jager, A., Sarno, J., Davis, K. (2021). Mass cytometry of hematopoietic cells. In C. Cobaleda, I. Sánchez-García (a cura di), Leukemia Stem Cells Methods and Protocols (pp. 65-76). Humana Press Inc. [10.1007/978-1-0716-0810-4_5].
Mass cytometry of hematopoietic cells
Sarno J.;
2021
Abstract
Mass cytometry is now a well-established method that enables the measurement of 40–50 markers (generally proteins but transcripts are also possible) in single cells. Analytes are detected via antibodies tagged with heavy metal and detected by using a time-of-flight mass spectrometer. Over the past decade, mass cytometry has proven to be a valuable method for immunophenotyping hematopoietic cells with remarkable precision in both healthy and malignant scenarios. This chapter explains in detail how to profile hematopoietic cells by using this high-dimensional multiplexed approach.
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