The regulation of chromatin structure depends on a dynamic, multiple mechanisms that modulate gene expression and constitute the epigenome. The Polycomb group (PcG) of proteins are epigenetic factors involved in the transcriptional repression. Among their multilevel, chromatin-associated functions, PcG proteins mediate the establishment and maintenance of higher-order structures at target genes, allowing the transmission of transcriptional programs throughout the cell cycle. In the nucleus, PcG proteins localize close to the pericentric heterochromatin forming microscopically foci, called Polycomb bodies. Here, to visualize the tissue-specific PcG distribution in the aorta, dorsal skin and hindlimb muscles, we combine a fluorescence-activated cell sorter (FACS)-based method with an immunofluorescence staining.

Rosti, V., Gorini, F., Santarelli, P., Sarnicola, M., Magnani, S., Lanzuolo, C. (2023). Polycomb Bodies Detection in Murine Fibromuscular Stroma from Skin, Skeletal Muscles, and Aortic Tissues. In C. Lanzuolo, F. Marasca (a cura di), Polycomb Group Proteins Methods and Protocols (pp. 125-146). Humana Press Inc. [10.1007/978-1-0716-3143-0_11].

Polycomb Bodies Detection in Murine Fibromuscular Stroma from Skin, Skeletal Muscles, and Aortic Tissues

Rosti V.;Santarelli P.;
2023

Abstract

The regulation of chromatin structure depends on a dynamic, multiple mechanisms that modulate gene expression and constitute the epigenome. The Polycomb group (PcG) of proteins are epigenetic factors involved in the transcriptional repression. Among their multilevel, chromatin-associated functions, PcG proteins mediate the establishment and maintenance of higher-order structures at target genes, allowing the transmission of transcriptional programs throughout the cell cycle. In the nucleus, PcG proteins localize close to the pericentric heterochromatin forming microscopically foci, called Polycomb bodies. Here, to visualize the tissue-specific PcG distribution in the aorta, dorsal skin and hindlimb muscles, we combine a fluorescence-activated cell sorter (FACS)-based method with an immunofluorescence staining.
Capitolo o saggio
Fresh tissue digestion; Immunofluorescence; Murine fibroblast characterization; Polycomb foci;
English
Polycomb Group Proteins Methods and Protocols
Lanzuolo, C; Marasca, F
2023
9781071631423
2655 MIMB
Humana Press Inc.
125
146
Rosti, V., Gorini, F., Santarelli, P., Sarnicola, M., Magnani, S., Lanzuolo, C. (2023). Polycomb Bodies Detection in Murine Fibromuscular Stroma from Skin, Skeletal Muscles, and Aortic Tissues. In C. Lanzuolo, F. Marasca (a cura di), Polycomb Group Proteins Methods and Protocols (pp. 125-146). Humana Press Inc. [10.1007/978-1-0716-3143-0_11].
none
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/503880
Citazioni
  • Scopus 0
  • ???jsp.display-item.citation.isi??? ND
Social impact