Light-sheet microscopy is a powerful method for imaging small translucent samples in vivo, owing to its unique combination of fast imaging speeds, large field of view, and low phototoxicity. This chapter briefly reviews state-of-the-art technology for variations of light-sheet microscopy. We review recent examples of optogenetics in combination with light-sheet microscopy and discuss some current bottlenecks and horizons of light sheet in all-optical physiology. We describe how 3-dimensional optogenetics can be added to an home-built light-sheet microscope, including technical notes about choices in microscope configuration to consider depending on the time and length scales of interest.
Maddalena, L., Pozzi, P., Ceffa, N., Hoeven, B., Carroll, E. (2023). Optogenetics and Light-Sheet Microscopy. In Eirini Papagiakoumou (ed.) (a cura di), All-Optical Methods to Study Neuronal Function (pp. 231-261). Humana Press Inc. [10.1007/978-1-0716-2764-8_8].
Optogenetics and Light-Sheet Microscopy
Pozzi, Paolo;Ceffa, Nicolò G.;
2023
Abstract
Light-sheet microscopy is a powerful method for imaging small translucent samples in vivo, owing to its unique combination of fast imaging speeds, large field of view, and low phototoxicity. This chapter briefly reviews state-of-the-art technology for variations of light-sheet microscopy. We review recent examples of optogenetics in combination with light-sheet microscopy and discuss some current bottlenecks and horizons of light sheet in all-optical physiology. We describe how 3-dimensional optogenetics can be added to an home-built light-sheet microscope, including technical notes about choices in microscope configuration to consider depending on the time and length scales of interest.
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