Germinal centers are short-lived microanatomical compartments with essential roles in adaptive immunity. These lymphoid structures can be identified in secondary lymphoid organs using both flow cytometry and immunohistological analyses, but only the latter provides useful architectural and spatial information. Here we describe how to use immunofluorescence and immunohistochemistry with specific antibodies to precisely highlight the cellular and architectural features of germinal centers, both in human and mouse secondary lymphoid organs, and to study their normal development and disturbance in disease.
Dominguez Sola, D., Cattoretti, G. (2017). Analysis of the germinal center reaction in tissue sections. In D. Calado (a cura di), Germinal Centers : Methods and Protocols (pp. 1-20). Humana Press Inc. [10.1007/978-1-4939-7095-7_1].
Analysis of the germinal center reaction in tissue sections
CATTORETTI, GIORGIOUltimo
2017
Abstract
Germinal centers are short-lived microanatomical compartments with essential roles in adaptive immunity. These lymphoid structures can be identified in secondary lymphoid organs using both flow cytometry and immunohistological analyses, but only the latter provides useful architectural and spatial information. Here we describe how to use immunofluorescence and immunohistochemistry with specific antibodies to precisely highlight the cellular and architectural features of germinal centers, both in human and mouse secondary lymphoid organs, and to study their normal development and disturbance in disease.
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