Overexpression of hybrid Virus-Like-Particles (VLP) was found to dramatically affect cell kinetics and morphology with a significant increase in doubling times, induction times and major alterations in cell volume distributions. Optimal expression (ca. 10 mg/l) was obtained with a two-step fermentation strategy (raffinose → galactose) coupled to GAL4 overexpression, resulting in partial cell lysis and VLP release.

Cavadini, P., Popolo, L., Vai, M., Varona, R., Garofano, L., Vanoni, M. (1994). In Saccharomyces cerevisiae overexpression of hybrid Virus-Like-Particles correlates with altered cell volume distributions. BIOTECHNOLOGY LETTERS, 16(11), 1131-1134 [10.1007/BF01020838].

In Saccharomyces cerevisiae overexpression of hybrid Virus-Like-Particles correlates with altered cell volume distributions

VAI, MARINA;VANONI, MARCO ERCOLE
1994

Abstract

Overexpression of hybrid Virus-Like-Particles (VLP) was found to dramatically affect cell kinetics and morphology with a significant increase in doubling times, induction times and major alterations in cell volume distributions. Optimal expression (ca. 10 mg/l) was obtained with a two-step fermentation strategy (raffinose → galactose) coupled to GAL4 overexpression, resulting in partial cell lysis and VLP release.
Articolo in rivista - Articolo scientifico
Virus-Like-Particles; two-step fermentation; GAL4 overexpression
English
1994
16
11
1131
1134
none
Cavadini, P., Popolo, L., Vai, M., Varona, R., Garofano, L., Vanoni, M. (1994). In Saccharomyces cerevisiae overexpression of hybrid Virus-Like-Particles correlates with altered cell volume distributions. BIOTECHNOLOGY LETTERS, 16(11), 1131-1134 [10.1007/BF01020838].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/99047
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