Soft X-ray Contact Microscopy (SXCM) of Caenorhabditis elegans nematodes with typical length similar to 800 mum and diameter similar to 30 mum has been performed using the PALS laser source of wavelength lambda = 1.314 mum and pulse duration tau ( FWHM) = 400 ps. Pulsed soft X-rays were generated using molybdenum and gold targets with laser intensities I greater than or equal to 10(14) W/cm(2). Images have been recorded on PMMA photo resists and analyzed using an atomic force microscope operating in contact mode. Cuticle features and several internal organs have been identified in the SXCM images including lateral field, cuticle annuli, pharynx, and hypodermal and neuronal cell nuclei.
Poletti, G., Orsini, F., Batani, D., Bernardinello, A., Desai, T., Ullschmied, J., et al. (2004). Soft X-ray contact microscopy of nematode Caenorhabditis elegans. THE EUROPEAN PHYSICAL JOURNAL. D, ATOMIC, MOLECULAR AND OPTICAL PHYSICS, 30(2), 235-241 [10.1140/epjd/e2004-00084-4].
Soft X-ray contact microscopy of nematode Caenorhabditis elegans
BATANI, DINO DIMITRI;ZULLINI, ALDO
2004
Abstract
Soft X-ray Contact Microscopy (SXCM) of Caenorhabditis elegans nematodes with typical length similar to 800 mum and diameter similar to 30 mum has been performed using the PALS laser source of wavelength lambda = 1.314 mum and pulse duration tau ( FWHM) = 400 ps. Pulsed soft X-rays were generated using molybdenum and gold targets with laser intensities I greater than or equal to 10(14) W/cm(2). Images have been recorded on PMMA photo resists and analyzed using an atomic force microscope operating in contact mode. Cuticle features and several internal organs have been identified in the SXCM images including lateral field, cuticle annuli, pharynx, and hypodermal and neuronal cell nuclei.
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