A photoelectric flame photometer is a device used in inorganic chemical analysis for determining the concentrations of certain metals in solution. It does this by measuring the intensity of the light emitted by the metal when the solution is sprayed under controlled conditions into a nonluminous flame. This protocol describes how to measure total cellular calcium (maximal emission at 622 nm, orange flame) in yeast using this technique.
Tisi, R., Martegani, E., Brandão, R. (2015). Total cellular Ca2+ measurements in yeast using flame photometry. COLD SPRING HARBOR PROTOCOLS, 2015(2), 214-216 [10.1101/pdb.prot076869].
Total cellular Ca2+ measurements in yeast using flame photometry
TISI, RENATA ANITA
Primo
;MARTEGANI, ENZOSecondo
;
2015
Abstract
A photoelectric flame photometer is a device used in inorganic chemical analysis for determining the concentrations of certain metals in solution. It does this by measuring the intensity of the light emitted by the metal when the solution is sprayed under controlled conditions into a nonluminous flame. This protocol describes how to measure total cellular calcium (maximal emission at 622 nm, orange flame) in yeast using this technique.
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