The helicase domain of the hepatitis C virus (HCV) protein NS3 is a major immunodominant region that elicits a strong and specific immune response in the majority of HCV infections. Use of NS3 helicase-derived antigens in immunometric diagnostic assays is a mainstay in detection of HCV infections. However, thermal instability of NS3 helicase-related polypeptides is a common and severe issue hampering reproducibility, performances and shelf-life of HCV diagnostic immunoassays. In this work, a novel NS3 helicase-derived antigen is presented, with a sensible improvement in thermal stability and diagnostic performances over the currently used NS3 helicase-related antigens.

(2009). Study of the hepatitis C virus NS3 helicase domain for application in a chemiluminescent immunoassay. (Tesi di dottorato, Università degli Studi di Milano-Bicocca, 2009).

Study of the hepatitis C virus NS3 helicase domain for application in a chemiluminescent immunoassay

REBUZZINI, GABRIELE
2009

Abstract

The helicase domain of the hepatitis C virus (HCV) protein NS3 is a major immunodominant region that elicits a strong and specific immune response in the majority of HCV infections. Use of NS3 helicase-derived antigens in immunometric diagnostic assays is a mainstay in detection of HCV infections. However, thermal instability of NS3 helicase-related polypeptides is a common and severe issue hampering reproducibility, performances and shelf-life of HCV diagnostic immunoassays. In this work, a novel NS3 helicase-derived antigen is presented, with a sensible improvement in thermal stability and diagnostic performances over the currently used NS3 helicase-related antigens.
PORRO, DANILO
DAL CORSO, ANDREA
HCV,NS3,helicase,hepatitis,virus,immunoassay,c33,thermal,stability,antigen
CHIM/11 - CHIMICA E BIOTECNOLOGIA DELLE FERMENTAZIONI
English
15-dic-2009
Scuola di dottorato di Scienze
BIOTECNOLOGIE INDUSTRIALI - 15R
22
2008/2009
Research performed in the Molecular Biology Lab at Diasorin S.p.A. (Nerviano, Italy)
open
(2009). Study of the hepatitis C virus NS3 helicase domain for application in a chemiluminescent immunoassay. (Tesi di dottorato, Università degli Studi di Milano-Bicocca, 2009).
File in questo prodotto:
File Dimensione Formato  
phd_unimib_033809.pdf

accesso aperto

Tipologia di allegato: Doctoral thesis
Dimensione 2.42 MB
Formato Adobe PDF
2.42 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/7477
Citazioni
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
Social impact