Speciation of particulate matter's organic fraction and its mechanis of action on human health

Three main researches have been employed for the implementation of a protocol analysis for the characterization and quantification of the lignin fraction in the particulate matter at the concentration matrix level; the implementation of different methods of analysis of the toxic interesting pollutants, Oxy-PAHs; Nitro-PAHs and the Bisphenol A, that together with the large set of performed analysis, allowed the characterization of some PM fractions in relation with Indoor and Outdoor concentrations, human exposure and Urban – Rural – Remote sites composition. At last an in silica method was developed for the research of the proteins involved in the interaction with the pollutants of interest, optimized on Bisphenol A because of its history and recent interaction study with the Nuclear Receptors. From the involved pathway the Blood Serine Proteases are used to test the accuracy and reproducibility of obtained Autodock4.0 and Dock4.0 data. The method results useful for research on the biological mechanism of action in relation with both matrix concentrations and in vivo and in vitro studies. The data predicted will be confirmed by NMR analysis. The newest docking program gives more and more reproducible data, accurate and empirically shaped on the domain problem, at last the experimental data had to confirm or not confirm the predictions.