A highly sensitive optical probe for the detection of activated neutrophils and Neutrophil Extracellular Traps (NETs) is reported. It is based on a triple-quenched, super-silent tri-branched probe that generates >20 fold increase in fluorescence upon cleavage. The probe was highly specific for human neutrophil elastase, a protease that mediates a variety of inflammatory diseases, and detected NETosis and neutrophil activation in in vitro differentiated neutrophils and isolated human neutrophils. This journal is
Rios, M., Garoffolo, G., Rinaldi, G., Megia-Fernandez, A., Ferrari, S., Robb, C., et al. (2021). A fluorogenic peptide-based smartprobe for the detection of neutrophil extracellular traps and inflammation. CHEMICAL COMMUNICATIONS, 57(1), 97-100 [10.1039/d0cc07028a].
A fluorogenic peptide-based smartprobe for the detection of neutrophil extracellular traps and inflammation
Garoffolo G.;
2021
Abstract
A highly sensitive optical probe for the detection of activated neutrophils and Neutrophil Extracellular Traps (NETs) is reported. It is based on a triple-quenched, super-silent tri-branched probe that generates >20 fold increase in fluorescence upon cleavage. The probe was highly specific for human neutrophil elastase, a protease that mediates a variety of inflammatory diseases, and detected NETosis and neutrophil activation in in vitro differentiated neutrophils and isolated human neutrophils. This journal is
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