The use of immune checkpoint blockade (ICI), such as anti-PD-(L)-1 antibodies, has emerged as an effective treatment for both locally-advanced and advanced NSCLC. The 3’ UTR of PD-L1 gene is disrupted in several tumor types, leading to a stabilized form of PD-L1 and high expression. microRNAs bind 3’ UTR of genes (including PD-L1) and induce mRNA degradation and translational repression. Here, we performed a high-throughput functional miRNA screening using human miRNA lentiviral library (shMIMIC) in NSCLC cells to investigate the role of miRNA in regulating PD-L1 expression and response to ICI treatment.
Cuttano, R., Afanga, M., Melocchi, V., Dama, E., Bizzarri, M., Carbonelli, C., et al. (2024). miRNome functional profiling of non-small cell lung cancer identified new mechanisms of PD-L1 regulation. In International Association for the Study of Lung Cancer (IASLC) 2024 World Conference On Lung Cancer.
miRNome functional profiling of non-small cell lung cancer identified new mechanisms of PD-L1 regulation
Afanga Miriam Kuku;Elisa Dama;
2024
Abstract
The use of immune checkpoint blockade (ICI), such as anti-PD-(L)-1 antibodies, has emerged as an effective treatment for both locally-advanced and advanced NSCLC. The 3’ UTR of PD-L1 gene is disrupted in several tumor types, leading to a stabilized form of PD-L1 and high expression. microRNAs bind 3’ UTR of genes (including PD-L1) and induce mRNA degradation and translational repression. Here, we performed a high-throughput functional miRNA screening using human miRNA lentiviral library (shMIMIC) in NSCLC cells to investigate the role of miRNA in regulating PD-L1 expression and response to ICI treatment.
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