Zinc is an essential cofactor for bacterial metabolism, and many Enterobacteriaceae express the zinc transporters ZnuABC and ZupT to acquire this metal in the host. However, the probiotic bacterium Escherichia coli Nissle 1917 (or “Nissle”) exhibits appreciable growth in zinc-limited media even when these transporters are deleted. Here, we show that Nissle utilizes the siderophore yersiniabactin as a zincophore, enabling Nissle to grow in zinc-limited media, to tolerate calprotectin-mediated zinc sequestration, and to thrive in the inflamed gut. We also show that yersiniabactin’s affinity for iron or zinc changes in a pH-dependent manner, with increased relative zinc binding as the pH increases. Thus, our results indicate that siderophore metal affinity can be influenced by the local environment and reveal a mechanism of zinc acquisition available to commensal and pathogenic Enterobacteriaceae.

Behnsen, J., Zhi, H., Aron, A., Subramanian, V., Santus, W., Lee, M., et al. (2021). Siderophore-mediated zinc acquisition enhances enterobacterial colonization of the inflamed gut. NATURE COMMUNICATIONS, 12(1) [10.1038/s41467-021-27297-2].

Siderophore-mediated zinc acquisition enhances enterobacterial colonization of the inflamed gut

Santus W.;
2021

Abstract

Zinc is an essential cofactor for bacterial metabolism, and many Enterobacteriaceae express the zinc transporters ZnuABC and ZupT to acquire this metal in the host. However, the probiotic bacterium Escherichia coli Nissle 1917 (or “Nissle”) exhibits appreciable growth in zinc-limited media even when these transporters are deleted. Here, we show that Nissle utilizes the siderophore yersiniabactin as a zincophore, enabling Nissle to grow in zinc-limited media, to tolerate calprotectin-mediated zinc sequestration, and to thrive in the inflamed gut. We also show that yersiniabactin’s affinity for iron or zinc changes in a pH-dependent manner, with increased relative zinc binding as the pH increases. Thus, our results indicate that siderophore metal affinity can be influenced by the local environment and reveal a mechanism of zinc acquisition available to commensal and pathogenic Enterobacteriaceae.
Articolo in rivista - Articolo scientifico
Animals; ATP-Binding Cassette Transporters; Bacterial Proteins; Carrier Proteins; Colon; Enterobacteriaceae; Escherichia coli; Escherichia coli Proteins; Female; Leukocyte L1 Antigen Complex; Membrane Transport Proteins; Mice; Mice, Inbred C57BL; Phenols; Salmonella typhi; Siderophores; Thiazoles; Zinc
English
2021
12
1
7016
open
Behnsen, J., Zhi, H., Aron, A., Subramanian, V., Santus, W., Lee, M., et al. (2021). Siderophore-mediated zinc acquisition enhances enterobacterial colonization of the inflamed gut. NATURE COMMUNICATIONS, 12(1) [10.1038/s41467-021-27297-2].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/504462
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