Aim: Cell microenvironment contains a plethora of information that influences cell modulation. Indeed, the extracellular matrix plays a central role in tissue development. Reproducing the cell–extracellular matrix crosstalk able to recapitulate both physical and biochemical signals is crucial to obtain functional tissue models or regenerative strategies. Materials & methods: Here, a combined method is proposed to easily functionalize collagen surface films, tailoring morphological properties. Oxygen nonthermal plasma treatment and glyco-conjugation with chondroitin sulfate are used to modify surface properties. Results: It results in higher adhesion, proliferation and morphological organization of U87 glioblastoma cells. Conclusion: Our finding suggests new promising strategies for the development of collagen-based biomaterials, which can be employed for advanced in vitro models.
Barbugian, F., Cadamuro, F., Nicotra, F., Riccardi, C., Russo, L. (2024). Plasma-Treated Collagen Functionalized With Chondroitin Sulfate as Bioactive and Nanostructured Extracellular Matrix Mimics. NANOMEDICINE, 19(9), 799-810 [10.2217/nnm-2023-0310].
Plasma-Treated Collagen Functionalized With Chondroitin Sulfate as Bioactive and Nanostructured Extracellular Matrix Mimics
Barbugian, Federica;Cadamuro, Francesca;Nicotra, Francesco;Riccardi, Claudia
;Russo, Laura
2024
Abstract
Aim: Cell microenvironment contains a plethora of information that influences cell modulation. Indeed, the extracellular matrix plays a central role in tissue development. Reproducing the cell–extracellular matrix crosstalk able to recapitulate both physical and biochemical signals is crucial to obtain functional tissue models or regenerative strategies. Materials & methods: Here, a combined method is proposed to easily functionalize collagen surface films, tailoring morphological properties. Oxygen nonthermal plasma treatment and glyco-conjugation with chondroitin sulfate are used to modify surface properties. Results: It results in higher adhesion, proliferation and morphological organization of U87 glioblastoma cells. Conclusion: Our finding suggests new promising strategies for the development of collagen-based biomaterials, which can be employed for advanced in vitro models.File | Dimensione | Formato | |
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