Here, we provide detailed protocols for the isolation of mouse Kupffer cells – the liver-resident macrophages – for phenotypic (e.g., via flow cytometry, mass cytometry, or RNA-sequencing) analyses or for functional experiments involving cell culture. The procedures presented can be adapted for the isolation of other hepatic cell populations. For complete details on the use and execution of this protocol, please refer to De Simone et al. (2021).

Andreata, F., Blériot, C., Di Lucia, P., De Simone, G., Fumagalli, V., Ficht, X., et al. (2021). Isolation of mouse Kupffer cells for phenotypic and functional studies. STAR PROTOCOLS, 2(4) [10.1016/j.xpro.2021.100831].

Isolation of mouse Kupffer cells for phenotypic and functional studies

Andreata, Francesco;
2021

Abstract

Here, we provide detailed protocols for the isolation of mouse Kupffer cells – the liver-resident macrophages – for phenotypic (e.g., via flow cytometry, mass cytometry, or RNA-sequencing) analyses or for functional experiments involving cell culture. The procedures presented can be adapted for the isolation of other hepatic cell populations. For complete details on the use and execution of this protocol, please refer to De Simone et al. (2021).
Articolo in rivista - Articolo scientifico
Cell culture; Cell isolation; Flow Cytometry/Mass Cytometry; Immunology; Single Cell;
English
2021
2
4
100831
none
Andreata, F., Blériot, C., Di Lucia, P., De Simone, G., Fumagalli, V., Ficht, X., et al. (2021). Isolation of mouse Kupffer cells for phenotypic and functional studies. STAR PROTOCOLS, 2(4) [10.1016/j.xpro.2021.100831].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/417581
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