OSTEOBIOL (R) EFFECT ON DENTAL PULP DERIVED STEM CELLS

OsteoBiol (R) is a porcine bone largely employed as substitute for bone grafting due to its good biocompatibility and osteoconductive properties. To better understand how cortical porcine bone can induce osteoblast differentiation and proliferation in mesenchymal stem cells, a specific study was carried out. The quantitative expression of mRNA of specific genes, like transcriptional factors (RUNX2 and SP7), bone related genes (SPP1, COL1A1, COL3A1, ALPL, and FOSL1) and mesenchymal stem cells marker (ENG) was examined by means of real time Reverse Transcription-Polymerase Chain. The activation of osteoblast related genes FOSL1, RUNX2 and SPP1 and the disappearance of the mesenchymal stem cells marker, ENG, make OsteoBiol (R) an ideal scaffold for bone regeneration in the restoring of skeletal defects