Specificity of substrate recognition by Pseudomonas fluorescens N3 dioxygenase. The role of the oxidation potential and molecular geometry

Pseudomonas fluorescens N3 is able to grow on naphthalene as the sole carbon and energy source, The mutant TTC1, blocked at the dihydrodiol dehydrogenase level, which can transform the hydrocarbon into the corresponding dihydrodiol, has been used to produce bioconversion products, To rationalize the different grades of conversion obtained with different substrates, a study was performed using non-naphthalene derivatives, including benzenes, conjugated benzenes, and polycyclic aromatic hydrocarbons, The corresponding diols obtained by bioconversion have been isolated and characterized, ia theoretical model that considers both energy and geometry factors has been proposed to rationalize the experimental data, Good agreement has been found between the calculated values and the experimental results