The introduction of metallic nanoparticles (mNPs) into the diet is a matter of concern for human health. In particular, their effect on the gastrointestinal tract may potentially lead to the increased passage of gluten peptides and the activation of the immune response. In consequence, dietary mNPs could play a role in the increasing worldwide celiac disease (CeD) incidence. We evaluated the potential synergistic effects that peptic-tryptic-digested gliadin (PT) and the most-used food mNPs may induce on the intestinal mucosa. PT interaction with mNPs and their consequent aggregation was detected by transmission electron microscopy (TEM) analyses and UV–Vis spectra. In vitro experiments on Caco-2 cells proved the synergistic cytotoxic effect of PT and mNPs, as well as alterations in the monolayer integrity and tight junction proteins. Exposure of duodenal biopsies to gliadin plus mNPs triggered cytokine production, but only in CeD biopsies. These results suggest that mNPs used in the food sector may alter intestinal homeostasis, thus representing an additional environmental risk factor for the development of CeD.

Mancuso, C., Re, F., Rivolta, I., Elli, L., Gnodi, E., Beaulieu, J., et al. (2021). Dietary nanoparticles interact with gluten peptides and alter the intestinal homeostasis increasing the risk of celiac disease. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 22(11) [10.3390/ijms22116102].

Dietary nanoparticles interact with gluten peptides and alter the intestinal homeostasis increasing the risk of celiac disease

Mancuso, C;Re, F;Rivolta, I;Gnodi, E;Barisani, D
2021

Abstract

The introduction of metallic nanoparticles (mNPs) into the diet is a matter of concern for human health. In particular, their effect on the gastrointestinal tract may potentially lead to the increased passage of gluten peptides and the activation of the immune response. In consequence, dietary mNPs could play a role in the increasing worldwide celiac disease (CeD) incidence. We evaluated the potential synergistic effects that peptic-tryptic-digested gliadin (PT) and the most-used food mNPs may induce on the intestinal mucosa. PT interaction with mNPs and their consequent aggregation was detected by transmission electron microscopy (TEM) analyses and UV–Vis spectra. In vitro experiments on Caco-2 cells proved the synergistic cytotoxic effect of PT and mNPs, as well as alterations in the monolayer integrity and tight junction proteins. Exposure of duodenal biopsies to gliadin plus mNPs triggered cytokine production, but only in CeD biopsies. These results suggest that mNPs used in the food sector may alter intestinal homeostasis, thus representing an additional environmental risk factor for the development of CeD.
Articolo in rivista - Articolo scientifico
Celiac disease; Food additives; Metallic nanoparticles; Nanotoxicity; Risk factors;
English
2021
22
11
6102
open
Mancuso, C., Re, F., Rivolta, I., Elli, L., Gnodi, E., Beaulieu, J., et al. (2021). Dietary nanoparticles interact with gluten peptides and alter the intestinal homeostasis increasing the risk of celiac disease. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 22(11) [10.3390/ijms22116102].
File in questo prodotto:
File Dimensione Formato  
Mancuso-2021-IJMolSci-VoR.pdf

accesso aperto

Descrizione: This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Tipologia di allegato: Publisher’s Version (Version of Record, VoR)
Licenza: Creative Commons
Dimensione 5.14 MB
Formato Adobe PDF
5.14 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/341378
Citazioni
  • Scopus 7
  • ???jsp.display-item.citation.isi??? 7
Social impact