Size-exclusion and ion-exchange high-performance liquid chromatography were used to monitor the presence of polymeric aggregates and of families of allergens of similar isoelectric point in Parietaria judaica pollen extracts. A radio-allergo-sorbent test and chromatofocusing were used for the analysis of individual fractions. This allows the detection of two groups of allergens, having pI of 4.7 and >5.7, respectively, and shows the effect of dialysis or ultrafiltration on the distribution of allergens.
Bassoli, A., Chioccara, F., Digregorio, G., Rindone, B., Tollari, S., Falagiani, P., et al. (1988). Analysis of allergenic components of a parietaria-judaica pollen extract by chromatographic methods for the evaluation of purification procedures. JOURNAL OF CHROMATOGRAPHY A, 444(C), 209-218 [10.1016/S0021-9673(01)94024-5].
Analysis of allergenic components of a parietaria-judaica pollen extract by chromatographic methods for the evaluation of purification procedures
RINDONE, BRUNO;BOLZACCHINI, EZIO
1988
Abstract
Size-exclusion and ion-exchange high-performance liquid chromatography were used to monitor the presence of polymeric aggregates and of families of allergens of similar isoelectric point in Parietaria judaica pollen extracts. A radio-allergo-sorbent test and chromatofocusing were used for the analysis of individual fractions. This allows the detection of two groups of allergens, having pI of 4.7 and >5.7, respectively, and shows the effect of dialysis or ultrafiltration on the distribution of allergens.
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
