Soft X-ray contact microscopy (SXCM), using a pulsed X-ray source, offers the possibility of imaging the ultrastructure of living biological systems at sub-100 nm resolution, We have developed a table-top pulsed plasma S-ray source for this application, generated by a large-volume XeCl laser, achieving a good conversion efficiency to 'water-window' X-rays (hv approximate to 280-530 eV). Optimum plasma conditions for SXCM are discussed, including the effect of pulse duration, target material and resist development time on image resolution. Soft X-ray contact images of Chlamydomonas dysosmos (unicellular alga) and of the cyanobacterium Leptolyngbya are shown.
Albertano, P., Reale, L., Palladino, L., Reale, A., Cotton, R., Bollanti, S., et al. (1997). X-ray contact microscopy using an excimer laser plasma source with different target materials and laser pulse durations. JOURNAL OF MICROSCOPY, 187(2), 96-103 [10.1046/j.1365-2818.1997.2120768.x].
X-ray contact microscopy using an excimer laser plasma source with different target materials and laser pulse durations
BATANI, DINO DIMITRI;MORET, MASSIMO;
1997
Abstract
Soft X-ray contact microscopy (SXCM), using a pulsed X-ray source, offers the possibility of imaging the ultrastructure of living biological systems at sub-100 nm resolution, We have developed a table-top pulsed plasma S-ray source for this application, generated by a large-volume XeCl laser, achieving a good conversion efficiency to 'water-window' X-rays (hv approximate to 280-530 eV). Optimum plasma conditions for SXCM are discussed, including the effect of pulse duration, target material and resist development time on image resolution. Soft X-ray contact images of Chlamydomonas dysosmos (unicellular alga) and of the cyanobacterium Leptolyngbya are shown.
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