Human transferrin (hTf) is an iron-binding glycoprotein (Mw 78 kDa) present in blood [1]. Two of its glycoforms, asialo- and disialo-hTF, named carbohydrate deficient transferrin (CDT), represent a well known biological marker of chronic alcohol abuse [2]. To determine CDT, one of the techniques used is capillary electrophoresis (CE). Since CE separations of macromolecules are spoiled by analyte interactions with the silica capillary, polysaccharides can be used as coatings to reduce these interactions [3]. Chitosan is a linear polysaccharide produced from chitin, by alkaline deacetylation [3]. It possesses reactive groups that can be exploited for production of derivatives, with increased solubility [4]. In the present work, chitosan derivatives were synthetized by chemical modification, including nucleophilic substitution, reductive amination, and Michael reaction. All chitosan derivatives were characterized by different techniques, including Nuclear Magnetic Resonance spectroscopy, Size Exclusion Chromatography-Triple Detector Array and Dynamic Light Scattering. The analysis highlighted a different chemical structure between chitosan and its derivatives. A preliminary evaluation of their application in CE showed effects on the streaming potential (an indirect parameter correlated with the interactions between capillary wall and electrolyte solution) with improvements in hTf peak resolution, in selected conditions. To conclude, polysaccharide derivatives have been successfully synthetized and characterized and a preliminary glimpse of their possible application to CE was given. References 1. Paterlini V.; Porpiglia N. M.; De Palo E. F.; Tagliaro F. Alcohol, 2019, Volum 78, 43. 2. Porpiglia N.M.; De Palo E. F.; Savchuk S. A.; Appolonova S. A.; Bortolotti F.; Tagliaro, Chim. Acta, 2018, Volum 486, 256. 3. Rinaudo M. Prog. Polym. Sci., 2006, Volum 31, 603. 4. Casettari L.; Vllasaliu S.; Castagnino E.; Stolnik S.; Howslw S.; Illum L. Prog. Polym. Sci, 2012, Volum 37, 659
Alessi, A., Porpiglia, N., Tagliaro, F., Musile, G., Pellegrini, B., Antonini, C., et al. (2021). Development pf polysaccharide derivatives for analytical applications in Capillary Electrophoresis. Intervento presentato a: Scuola Avanzata di Chimica dei Carboidrati (XVII CSCC 2021), Certosa di Pontignano (Pontignano, Siena, Italia).
Development pf polysaccharide derivatives for analytical applications in Capillary Electrophoresis
Antonini, C;Tagliaro, I;Russo, L;
2021
Abstract
Human transferrin (hTf) is an iron-binding glycoprotein (Mw 78 kDa) present in blood [1]. Two of its glycoforms, asialo- and disialo-hTF, named carbohydrate deficient transferrin (CDT), represent a well known biological marker of chronic alcohol abuse [2]. To determine CDT, one of the techniques used is capillary electrophoresis (CE). Since CE separations of macromolecules are spoiled by analyte interactions with the silica capillary, polysaccharides can be used as coatings to reduce these interactions [3]. Chitosan is a linear polysaccharide produced from chitin, by alkaline deacetylation [3]. It possesses reactive groups that can be exploited for production of derivatives, with increased solubility [4]. In the present work, chitosan derivatives were synthetized by chemical modification, including nucleophilic substitution, reductive amination, and Michael reaction. All chitosan derivatives were characterized by different techniques, including Nuclear Magnetic Resonance spectroscopy, Size Exclusion Chromatography-Triple Detector Array and Dynamic Light Scattering. The analysis highlighted a different chemical structure between chitosan and its derivatives. A preliminary evaluation of their application in CE showed effects on the streaming potential (an indirect parameter correlated with the interactions between capillary wall and electrolyte solution) with improvements in hTf peak resolution, in selected conditions. To conclude, polysaccharide derivatives have been successfully synthetized and characterized and a preliminary glimpse of their possible application to CE was given. References 1. Paterlini V.; Porpiglia N. M.; De Palo E. F.; Tagliaro F. Alcohol, 2019, Volum 78, 43. 2. Porpiglia N.M.; De Palo E. F.; Savchuk S. A.; Appolonova S. A.; Bortolotti F.; Tagliaro, Chim. Acta, 2018, Volum 486, 256. 3. Rinaudo M. Prog. Polym. Sci., 2006, Volum 31, 603. 4. Casettari L.; Vllasaliu S.; Castagnino E.; Stolnik S.; Howslw S.; Illum L. Prog. Polym. Sci, 2012, Volum 37, 659
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
