A selective oxygen biosensor based on bilirubin oxidase (BOx) was developed. The sensor was used for determining oxygen profiles in a membraneless, single-chamber microbial fuel cell (SCMFC), fed with raw wastewater. The linear response of the sensor was optimized by a diffusion layer of silica gel. A computer-controlled stage was used to obtain accurate and precise measurements. Oxygen concentration in biofilms covering electrodes was measured, showing 3mgL-1 of O2 in the bulk solution, decreasing to 0mgL-1 in the cathodic biofilm. The MFC generated power in the range of 0-0.08mW, associated to the oxygen content.
Grattieri, M., Babanova, S., Santoro, C., Guerrini, E., Trasatti, S., Cristiani, P., et al. (2015). Enzymatic Oxygen Micro-sensor based on Bilirubin Oxidase applied to Microbial Fuel Cells analysis. ELECTROANALYSIS, 27(2), 327-335 [10.1002/elan.201400543].
Enzymatic Oxygen Micro-sensor based on Bilirubin Oxidase applied to Microbial Fuel Cells analysis
Santoro C;
2015
Abstract
A selective oxygen biosensor based on bilirubin oxidase (BOx) was developed. The sensor was used for determining oxygen profiles in a membraneless, single-chamber microbial fuel cell (SCMFC), fed with raw wastewater. The linear response of the sensor was optimized by a diffusion layer of silica gel. A computer-controlled stage was used to obtain accurate and precise measurements. Oxygen concentration in biofilms covering electrodes was measured, showing 3mgL-1 of O2 in the bulk solution, decreasing to 0mgL-1 in the cathodic biofilm. The MFC generated power in the range of 0-0.08mW, associated to the oxygen content.File | Dimensione | Formato | |
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