The effect of some flavonoids on the K+-dependent and K+-independent leucine uptake into brush border membrane vesicles fromBombyx morilarval midgut was investigated. Among the compounds tested, cyclochampedol, recently purified fromArtocarpus champeden,was able to inhibit in micromolar range the leucine transport. The inhibition occurred both in the absence and in the presence of potassium and was not affected by leucine concentration. The apparentKiwas 0.25 mM. Cyclochampedol represents the first non-competitive inhibitor of an amino acid transport system in Lepidoptera. The relevance of this result is discussed.
Parenti, P., Pizzigoni, A., Hanozet, G., Hakim, E., Makmur, L., Achmad, S., et al. (1998). A new prenylated flavone from Arctocarpus champeden inhibits the K+-dependent amino acid transport in Bombyx mori midgut. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 244(2), 445-448 [10.1006/bbrc.1998.8299].
A new prenylated flavone from Arctocarpus champeden inhibits the K+-dependent amino acid transport in Bombyx mori midgut
PARENTI, PAOLO;
1998
Abstract
The effect of some flavonoids on the K+-dependent and K+-independent leucine uptake into brush border membrane vesicles fromBombyx morilarval midgut was investigated. Among the compounds tested, cyclochampedol, recently purified fromArtocarpus champeden,was able to inhibit in micromolar range the leucine transport. The inhibition occurred both in the absence and in the presence of potassium and was not affected by leucine concentration. The apparentKiwas 0.25 mM. Cyclochampedol represents the first non-competitive inhibitor of an amino acid transport system in Lepidoptera. The relevance of this result is discussed.
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