Application of laser in periodontology: Microbiological evaluation with PCR-real-time

In the last years,investigators have been seeking to comprehend the effectiveness of laser application in periodontal therapy,since it seems to have a high clinical predicability and it is welcome for the patients. The aim of the work is to evaluate the clinical efficiency of Nd:Yag laser by using PCR real time methodologies. The results suggest that laser applications in Periodontology are interesting in order to obtain sterilization of periodontal pockets and improvement of periodontal records With this work it is possible to evaluate the efficiency of Nd:Yag laser with and without antiseptic substances in not surgical periodontal therapy by using PCR real time methodologies. To verify the bactericidal ability of Nd:Yag laser therapy, the total bacterial amount and the presence of some important microbiota involved in periodontal disease (Porphyromonas gingivalis,Actinobacillus actinomycetemcomitans,Prevotella intermedia)are analyzed by molecular method (PCR Real Time)