Aging is a universal phenomenon involving the whole body and is characterized by metabolic and physiological decline, leading to cardiovascular defects and heart failure. To characterize the molecular basis of physiological cardiac aging, the proteomic profiles of Sprague Dawley rat hearts of 6, 22 and 30 months were analysed by DIGE and immunoblotting. Results indicate changes in myosin binding protein C, aldehyde dehydrogenase, serpins and sirtuin-3 which protects from the opening of the mitochondrial permeability transition pore induced by cyclophilin D increment. Conversely, an increase of fusion, a decrease of mitochondrial fission and the activation of the non-canonical autophagy pathway were observed. These results support the hypothesis of successful aging in this rat model.

Capitanio, D., Leone, R., Fania, C., Torretta, E., Gelfi, C. (2016). Sprague Dawley rats: A model of successful heart aging. EUPA OPEN PROTEOMICS, 12, 22-30 [10.1016/j.euprot.2016.03.017].

Sprague Dawley rats: A model of successful heart aging

Fania, Chiara;
2016

Abstract

Aging is a universal phenomenon involving the whole body and is characterized by metabolic and physiological decline, leading to cardiovascular defects and heart failure. To characterize the molecular basis of physiological cardiac aging, the proteomic profiles of Sprague Dawley rat hearts of 6, 22 and 30 months were analysed by DIGE and immunoblotting. Results indicate changes in myosin binding protein C, aldehyde dehydrogenase, serpins and sirtuin-3 which protects from the opening of the mitochondrial permeability transition pore induced by cyclophilin D increment. Conversely, an increase of fusion, a decrease of mitochondrial fission and the activation of the non-canonical autophagy pathway were observed. These results support the hypothesis of successful aging in this rat model.
Articolo in rivista - Articolo scientifico
2-D DIGE; Animal model; Heart proteome; Mass spectrometry; Successful aging;
2-D DIGE; Animal model; Heart proteome; Mass spectrometry; Successful aging
English
2016
12
22
30
none
Capitanio, D., Leone, R., Fania, C., Torretta, E., Gelfi, C. (2016). Sprague Dawley rats: A model of successful heart aging. EUPA OPEN PROTEOMICS, 12, 22-30 [10.1016/j.euprot.2016.03.017].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/215245
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