Recent studies suggest that human tumors are generated from cancer cells with stem cell (SC) properties. Spontaneously occurring cancers in dogs contain a diversity of cells that like for human tumors suggest that certain canine tumors are also generated from cancer stem cells (CSCs). CSCs, like normal SCs, have the capacity for self-renewal as mammospheres in suspension cultures. To understand how cells with SC properties contribute to canine mammary gland tumor development and progression, comparative analysis between normal SCs and CSCs, obtained from the same individual, is essential. We have utilized the property of sphere formation to develop culture conditions for propagating stem/progenitor cells from canine normal and tumor tissue. We show that cells from dissociated mammospheres retain sphere reformation capacity for several serial passages and have the capacity to generate organoid structures ex situ. Utilizing various culture conditions for passaging SCs and CSCs, fibroblast growth factor 2 (FGF2) and epidermal growth factor (EGF) were found to positively or negatively regulate mammosphere regeneration, organoid formation, and multi-lineage differentiation potential. The response of FGF2 and EGF on SCs and CSCs was different, with increased FGF2 and EGF self-renewal promoted in SCs and repressed in CSCs. Our protocol for propagating SCs from normal and tumor canine breast tissue will provide new opportunities in comparative mammary gland stem cell analysis between species and anticancer treatment and therapies for dogs. J. Cell. Biochem. 118: 570–584, 2017. © 2016 Wiley Periodicals, Inc

Cocola, C., Molgora, S., Piscitelli, E., Veronesi, M., Greco, M., Bragato, C., et al. (2017). FGF2 and EGF Are Required for Self-Renewal and Organoid Formation of Canine Normal and Tumor Breast Stem Cells. JOURNAL OF CELLULAR BIOCHEMISTRY, 118(3), 570-584 [10.1002/jcb.25737].

FGF2 and EGF Are Required for Self-Renewal and Organoid Formation of Canine Normal and Tumor Breast Stem Cells

Bragato, Cinzia;MILANESI, LUCIANO;
2017

Abstract

Recent studies suggest that human tumors are generated from cancer cells with stem cell (SC) properties. Spontaneously occurring cancers in dogs contain a diversity of cells that like for human tumors suggest that certain canine tumors are also generated from cancer stem cells (CSCs). CSCs, like normal SCs, have the capacity for self-renewal as mammospheres in suspension cultures. To understand how cells with SC properties contribute to canine mammary gland tumor development and progression, comparative analysis between normal SCs and CSCs, obtained from the same individual, is essential. We have utilized the property of sphere formation to develop culture conditions for propagating stem/progenitor cells from canine normal and tumor tissue. We show that cells from dissociated mammospheres retain sphere reformation capacity for several serial passages and have the capacity to generate organoid structures ex situ. Utilizing various culture conditions for passaging SCs and CSCs, fibroblast growth factor 2 (FGF2) and epidermal growth factor (EGF) were found to positively or negatively regulate mammosphere regeneration, organoid formation, and multi-lineage differentiation potential. The response of FGF2 and EGF on SCs and CSCs was different, with increased FGF2 and EGF self-renewal promoted in SCs and repressed in CSCs. Our protocol for propagating SCs from normal and tumor canine breast tissue will provide new opportunities in comparative mammary gland stem cell analysis between species and anticancer treatment and therapies for dogs. J. Cell. Biochem. 118: 570–584, 2017. © 2016 Wiley Periodicals, Inc
Articolo in rivista - Articolo scientifico
ACINI; BREAST; CANCER; CANINES; FGF2; MAMMARY; MAMMOSPHERE; ORGANOIDS; STEM; Animals; Cell Proliferation; Dogs; Epidermal Growth Factor; Female; Fibroblast Growth Factor 2; Mammary Neoplasms, Animal; Neoplastic Stem Cells; Organoids; Tumor Cells, Cultured; Biochemistry; Molecular Biology; Cell Biology
English
2017
118
3
570
584
reserved
Cocola, C., Molgora, S., Piscitelli, E., Veronesi, M., Greco, M., Bragato, C., et al. (2017). FGF2 and EGF Are Required for Self-Renewal and Organoid Formation of Canine Normal and Tumor Breast Stem Cells. JOURNAL OF CELLULAR BIOCHEMISTRY, 118(3), 570-584 [10.1002/jcb.25737].
File in questo prodotto:
File Dimensione Formato  
Cocola_et_al-2017-Journal_of_Cellular_Biochemistry (1).pdf

Solo gestori archivio

Tipologia di allegato: Publisher’s Version (Version of Record, VoR)
Dimensione 3.98 MB
Formato Adobe PDF
3.98 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/204561
Citazioni
  • Scopus 27
  • ???jsp.display-item.citation.isi??? 27
Social impact