Therapy with cyclosporin A (CsA) for immunosuppression after organ transplantation requires monitoring of its levels in blood owing to the narrow therapeutic index of the drug and to the high inter-individual variability of the drug absorption and metabolism. We describe the preparation of CsA labelled with stable isotopes ((13)C and (2)H) with an isotopic enrichment of about 99% using labelled glucose and its use as internal standard for quantification of CsA blood levels by isotope dilution/electrospray ionization mass spectrometry. The method was found to be linear in the tested range (1-1000 ng) with and without the matrix. The accuracy of the bracketting calibration curves prepared using 100 ng ml(-1) labelled CsA was within +/-1.7% (bias). The results confirmed the usefulness of the procedure as a reference method for the external quality assessment of the field methods for the evaluation of CsA blood concentration, the imprecision (relative standard deviation) and accuracy (bias) being <2%.

Magni, F., Pereira, S., Leoni, M., Grisenti, G., Kienle, M. (2001). Quantitation of cyclosporin A in whole blood by liquid chromatography/stable isotope dilution electrospray ionization mass spectrometry. JOURNAL OF MASS SPECTROMETRY, 36(6), 670-676 [10.1002/jms.169].

Quantitation of cyclosporin A in whole blood by liquid chromatography/stable isotope dilution electrospray ionization mass spectrometry

MAGNI, FULVIO;KIENLE, MARZIA DONATELLA
2001

Abstract

Therapy with cyclosporin A (CsA) for immunosuppression after organ transplantation requires monitoring of its levels in blood owing to the narrow therapeutic index of the drug and to the high inter-individual variability of the drug absorption and metabolism. We describe the preparation of CsA labelled with stable isotopes ((13)C and (2)H) with an isotopic enrichment of about 99% using labelled glucose and its use as internal standard for quantification of CsA blood levels by isotope dilution/electrospray ionization mass spectrometry. The method was found to be linear in the tested range (1-1000 ng) with and without the matrix. The accuracy of the bracketting calibration curves prepared using 100 ng ml(-1) labelled CsA was within +/-1.7% (bias). The results confirmed the usefulness of the procedure as a reference method for the external quality assessment of the field methods for the evaluation of CsA blood concentration, the imprecision (relative standard deviation) and accuracy (bias) being <2%.
Articolo in rivista - Articolo scientifico
Spectrometry, Mass, Electrospray Ionization; Chromatography, Liquid; Cyclosporine; Chromatography, High Pressure Liquid; Immunosuppressive Agents; Solutions; Humans
English
2001
36
6
670
676
none
Magni, F., Pereira, S., Leoni, M., Grisenti, G., Kienle, M. (2001). Quantitation of cyclosporin A in whole blood by liquid chromatography/stable isotope dilution electrospray ionization mass spectrometry. JOURNAL OF MASS SPECTROMETRY, 36(6), 670-676 [10.1002/jms.169].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/16224
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