We previously identified TEL/ARG as a novel fusion transcript consisting of the oligomerization domain of TEL and the kinase domain of ARG, in a case of acute myeloid leukemia. We report here the existence of an alternatively spliced TEL/ARG transcript lacking part of a F-actin binding domain of ARG, and the phenotype of TEL/ARG expressing 293T cells. In 293T cells, both TEL/ARG forms co-localized with the cellular beta-actin and were associated with a morphologic change of the cells, consisting in cell rounding and detachment from the tissue culture plastic. We identified the Rho inhibitor p190RhoGAP, a critical regulator of cellular adhesion, as a target of the aberrant kinase.
Palmi, C., Fazio, G., Cassetti, A., Aloisi, A., Villa, A., Biondi, A., et al. (2006). TEL/ARG induces cytoskeletal abnormalities in 293T cells. CANCER LETTERS, 241(1), 79-86 [10.1016/j.canlet.2005.10.017].
TEL/ARG induces cytoskeletal abnormalities in 293T cells
PALMI, CHIARA;FAZIO, GRAZIA;VILLA, ANTONELLO;BIONDI, ANDREA;Cazzaniga, G.
2006
Abstract
We previously identified TEL/ARG as a novel fusion transcript consisting of the oligomerization domain of TEL and the kinase domain of ARG, in a case of acute myeloid leukemia. We report here the existence of an alternatively spliced TEL/ARG transcript lacking part of a F-actin binding domain of ARG, and the phenotype of TEL/ARG expressing 293T cells. In 293T cells, both TEL/ARG forms co-localized with the cellular beta-actin and were associated with a morphologic change of the cells, consisting in cell rounding and detachment from the tissue culture plastic. We identified the Rho inhibitor p190RhoGAP, a critical regulator of cellular adhesion, as a target of the aberrant kinase.
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