BACKGROUND: Oral squamous cell carcinoma (OSCC) represents the sixth most diffused cancer in developed countries. Mitogen-activated protein kinases (MAPKs) are proteins which transduce a vast array of extracellular signals into intracellular responses. The role of MAPK signalling pathway in cancer is not completely understood. MATERIALS AND METHODS: In this study, we attempted to specifically evaluate the activation state of MAPK in OSCC. MAPK expression and activation were analyzed by immunoblotting in thirty tissue samples of OSCC and their paired nonneoplastic perilesional tissues. On the same tissues, the activation and expression of MAPK JNK/SAPK were also evaluated by ELISA assay. RESULTS: Analyzing the levels of phospho-ERK1/2(p44/p42), a statistically significant reduction was observed in tumors compared to normal tissues. No statistically significant difference between tumor and control tissue was found for p38MAPK or JNK/SAPK. CONCLUSION: These results suggest that a reduction in ERK1/2(p44/p42) phosphorylation is correlated with tumor growth in OSCC.

Background: Oral squamous cell carcinoma (OSCC) represents the sixth most diffused cancer in developed countries. Mitogen-activated protein kinases (MAPKs) are proteins which transduce a vast array of extracellular signals into intracellular responses. The role of MAPK signalling pathway in cancer is not completely understood. Materials and Methods: In this study, we attempted to specifically evaluate the activation state of MAPK in OSCC. MAPK expression and activation were analyzed by immunoblotting in thirty tissue samples of OSCC and their paired nonneoplastic perilesional tissues. On the same tissues, the activation and expression of MAPK JNK/SAPK were also evaluated by ELISA assay. Results: Analyzing the levels of phospho-ERK1/2p44/p42, a statistically significant reduction was observed in tumors compared to normal tissues. No statistically significant difference between tumor and control tissue was found for p38MAPK or JNK/SAPK. Conclusion: These results suggest that a reduction in ERK1/2p44/p42 phosphorylation is correlated with tumor growth in OSCC

Aguzzi, A., Maggioni, D., Nicolini, G., Tredici, G., Gaini, R., Garavello, W. (2009). MAP kinase modulation in squamous cell carcinoma of the oral cavity. ANTICANCER RESEARCH, 29(1), 303-308.

MAP kinase modulation in squamous cell carcinoma of the oral cavity

MAGGIONI, DANIELE;NICOLINI, GABRIELLA;TREDICI, GIOVANNI;GAINI, RENATO MARIA;GARAVELLO, WERNER
2009

Abstract

Background: Oral squamous cell carcinoma (OSCC) represents the sixth most diffused cancer in developed countries. Mitogen-activated protein kinases (MAPKs) are proteins which transduce a vast array of extracellular signals into intracellular responses. The role of MAPK signalling pathway in cancer is not completely understood. Materials and Methods: In this study, we attempted to specifically evaluate the activation state of MAPK in OSCC. MAPK expression and activation were analyzed by immunoblotting in thirty tissue samples of OSCC and their paired nonneoplastic perilesional tissues. On the same tissues, the activation and expression of MAPK JNK/SAPK were also evaluated by ELISA assay. Results: Analyzing the levels of phospho-ERK1/2p44/p42, a statistically significant reduction was observed in tumors compared to normal tissues. No statistically significant difference between tumor and control tissue was found for p38MAPK or JNK/SAPK. Conclusion: These results suggest that a reduction in ERK1/2p44/p42 phosphorylation is correlated with tumor growth in OSCC
Articolo in rivista - Articolo scientifico
MAPK, oral cancer, squamous cell carcinoma, immunoblotting, phospho-ERK
MAPK, oral cancer, squamous cell carcinoma,immunoblotting, phospho-ERK
English
2009
29
1
303
308
none
Aguzzi, A., Maggioni, D., Nicolini, G., Tredici, G., Gaini, R., Garavello, W. (2009). MAP kinase modulation in squamous cell carcinoma of the oral cavity. ANTICANCER RESEARCH, 29(1), 303-308.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/11758
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